The purpose of this study is to confirm the mediating effect of leisure satisfaction in the effect of working mothers’ work-family conflict on their quality of life. The research subject of the research method was a survey of 207 working mothers. In order to verify the research model, demographic analysis, exploratory factor analysis, Cronbach’s α reliability, and Person’s correlation were analyzed using SPSS, and mediating effect verification was conducted using PROCESS Macro’s Model 4. First, the work-family conflict showed a significant negative correlation with leisure satisfaction and quality of life, and leisure satisfaction and quality of life showed a significant positive correlation. Second, it was found that the work-family conflict had a statistically significant negative effect on the quality of life and leisure satisfaction, and leisure satisfaction had a statistically significant and positive effect on the quality of life. Third, leisure satisfaction was found to have a statistically significant partial mediating effect in the effect of working mothers’ work-family conflict on the quality of life. It is intended to provide practical and policy implications to reduce the deterioration of quality of life due to the work-family conflict of working mothers.